Contract Research Organization
Alfa CDMO's CRO offers custom services for research, optimization, and development phases of your project.
- Gene synthesis, vector construction.
- Transient expression 6-8 weeks, 0-500 mg proteins.
- Antibody / Bispecific Antibody / Fusion protein / Antigen.
- Start with sequence of the mouse-origin monoclonal antibody, design, construct, screening.
- Short time (2 months) and higher binding affinity.
Antibody Fc Engineering
- ADCC increase / weaken
- CDC increase / weaken
- ADCP increase / weaken
- FcRn binding increase / weaken
- Antibody design for ADC
Design and Synthesis of Linker-Payloads
- A library of ready-for-use Linker-Payloads for rapid ADC screening.
- Design and synthesis of customized Linker-Payloads.
- Synthesis process development of Linker-Payloads and related precursors.
- Milligram to gram scale supply of Linker-Payloads and precursors.
ANTIBODY DRUG CONJUGATION SCREENING
ADC Conjugation Service
We are very familiar with the linkage strategy:
- Lysine-based conjugation
- Cysteine-based conjugation (Single-thiol conjugation /Double-thiol covalent conjugation)
- Site-specific conjugation
We have rich experience for different antibody & different linker-payloads:
- For different types of antibody (IgG1/ IgG2 / IgG4 / Bi-specific antibody)
- For different kinds of Linker- Payloads
ADC Assay and Mechanism Study
ADC analysis (For early stage)
- DAR / Free drug ratio / Concentration / Purity etc.
ADC Mechanism Study (For early stage)
- Tumor inhibition potency evaluation assay
- Binding affinity activity assay
- Antigen expression level detection
- Endocytosis activity assay
- Stability in serum assay
- Animal experiment (Tumor Models /MTD/PK/PD— mouse/rat)